Transgenic Animal Core Facility at MU

Now TAC provides chromosome counting services for cells of any mammalian species growing in a culture! [More Information]

Biomolecular Research Facilities
University of Missouri-Columbia

Transgenic Animal Core (TAC)

TAC Services Chromosome counting Generation of transgenic mice on FVB and c57Bl6 strains using client's DNA Generation of transgenic rats on SD strain using client's DNA Electroporation of KO and KI target constructs into ES cells and antibiotic selection of ES clones for recombinant event Screening of ES clones for homologous recombinant via PCR Blastocyst injection of ES cells and embryo transfer of micromanipulated embryos into recipient mice Expansion of ES clones Genotyping assays Customized projects. If the above services do not cover your needs, please, contact TAC to find out if TAC can help you by providing our customized services. Please complete the Upcoming TAC services survey!
Pronuclear Injection	Pronuclear Injection: Transgenic Mice and Rats Transgenic construct design and DNA purification guidelines Environmental Health & Safety/Biosafety Committee Production of transgenic mice and rats by pronuclear injection Selection of Mouse or Rat Strains PCR screening of trangenic offspring Health monitoring MU Animal Care and Use Committee
Chimeric Offspring	Embryonic Stem Cell Targeting Overview of services Investigator responsibilities Cell line selection Use of Gene Trap ES cell lines Construct design Electroporation and selection of ES cells Expansion of ES clones Blastocyst microinjection/embryo transfer Health monitoring/transfer of chimeric offspring
	Service Request Procedures Project submission for clients within MU system Transgenic Mouse or Transgenic Rat Projects Submission Submission of DNA for Electroporation Request for ES Screening via PCR Clone Submissions for Blastocyst Injections MTA form for external clients

Location:
E122/120 Veterinary Medicine Bldg.
University of Missouri-Columbia
Columbia, MO 65211

Phone/Fax:
(573) 884-0833
FAX: (573) 884-8211

About our facility

Staff /
contact information

Pronuclear injection:
Transgenic mice

Embryonic stem cell targeting

Transgenic construct design
and DNA purification guidelines

Service request
procedures

Forms

General timeline
for pronuclear
injections

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