Laser Scanning Confocal Microscopes Zeiss LSM 510 META NLO Inverted confocal microscope with visible (458, 477, 488, 514, 543, 633 nm) and two-photon lasers capable of spectral separation, tiling, FRET, FRAP. Equipped with heated stage and perfusion system for live cell imaging. BioRad Radiance 2000 on Olympus IX-70 microscope Inverted confocal microscope with 488, 568, and 637 nm lasers. Zeiss LSM 5 LIVE Ultra-fast inverted confocal microscope capable of up to 120 full frames/sec and 70 z-planes/sec. Equipped with incubator chamber for CO2 and temperature control during extended live cell imaging. Capable of automated tiled imaging of large volumes. Widefield Microscopes Leica DMI4000B with integrated Eppendorf Microinjection System Inverted microscope with brightfield, fluorescence, and fully automated DIC and phase contrast capabilities. Image capture with 1.4 megapixel, 12-bit, cooled CCD camera from Q-Imaging. Olympus IX70 inverted microscope with Orca digital camera. System includes motorized z-drive, wide array of filter sets and objectives, and is equipped with a Hamamatsu ORCA ER CCD camera. Leica MZFLIII Stereoscope with Optronics digital camera Capable of widefield fluorescence and brightfield. Equipped with Optronics 12-bit CCD camera and MagnaFire acquisition software Nikon Optiphot upright microscope with Spot2 digital camera. Capable of brightfield, fluorescence, phase contrast, darkfield, and DIC/Nomarski. Laser Capture Microdissection Molecular Devices ArcturusXT Laser Capture Microdissection System Microdissection/capture of small regions from tissue samples using UV/IR lasers. Capable of brightfield, phase contrast, DIC/Nomarski, and fluorescence with Nikon Eclipse TE2000 inverted microscope. Image acquisition with color CCD camera. Image Analysis/Processing Molecular Devices Metamorph: image analysis, object counting, morphological analysis, thresholding, 3D reconstruction/rotation of confocal optical sections. Bitplane Imaris:  3D reconstruction of optical sections, measurements in 3D, isosurfacing, animations, colocalization analysis. Media Cybernetics AutoDeblur: blind 3D and 2D image deconvolution with batch mode. Adobe Photoshop: image processing and editing with batch mode. Sample Preparation Intavis InsituPro VS: automated, streamlined in-situ hybridization protocols. Can process up to 60 slides per run or 96 whole-mount tissue samples per run. Recovery option available for valuable reagents. Reichert Ultracut S Ultramicrotome for cutting semi-thin & thin plastic sections for light and electron microscopy Paraffin Microtome for cutting 1-50 µm thick paraffin sections Leica Cryostat for cutting 2-100 µm thick frozen sections Invitrogen Supply Center The Cytology Core has an on-site Invitrogen Supply Center stocked with a wide variety of fluorescent secondary antibodies and markers. In addition to our normal inventory, clients may special order anything from the Invitrogen catalog for no additional cost and without shipping fees. File Transfer System The Cytology Core has a server that can be remotely accessed from any computer on the MU campus.  It serves as a TEMPORARY holding place for files to facilitate transfer of the data you generate at the Core to a local computer in your lab/office.  Please note that due to space limitations the storage space is cleared every morning at 8:00 am, such that your files will only be stored for a maximum of 24 hours and you need to transfer them to your computer on the same day they were placed on the server. For instructions on connecting to this utility contact MCC staff.