Price List
The Molecular Cytology Core is a campus-wide resource for light microscopy, immunocytochemistry and in situ hybridization techniques.  The instruments and equipment in the MCC are available for all to use on a per hour billing schedule.  We encourage users to become proficient enough to use the instruments independently, so most users go through an initial training session.  The length of the training session depends on the instrument, application, and level of experience of the user; times below are estimates for basic training of an inexperienced user. In addition, we offer assisted use during which Core staff will operate the instrument.  All assisted use and training is $30/hour plus the base usage fee for the instrument.
Zeiss 510 META NLO laser scanning confocal microscope
458 nm, 476 nm, 488 nm, 514 nm, 547 nm, 633 nm laser excitation lines plus variable wavelength IR laser (Coherent-Chameleon) for multiphoton imaging and UV excitation
• Base usage fee for trained clients	$30.00 / hr
• Initial training (~1 hr) or assisted use	$60.00 / hr
Zeiss LSM 5 LIVE
Ultra-fast inverted confocal microscope capable of up to 120 full frames/sec and 70 z-planes/sec. Equipped with 405, 488, 532 and 635 nm laser lines.
• Base usage fee for trained clients	$20.00 / hr
• Initial training (~1 hr) or assisted use	$50.00 / hr
BioRad Radiance 2000 laser scanning confocal microscope
488 nm, 568 nm, and 637 nm laser excitation lines
• Base usage fee for trained clients	$20.00 / hr
• Initial training (~1 hr) or assisted use	$50.00 / hr
Olympus IX70 inverted widefield fluorescence microscope
Photometrics Sensys 12-bit CCD camera, motorized z-drive, Metamorph image acquisition and analysis program.
• Base usage fee for trained clients	$5.00 / hr
• Initial training (~1 hr) or assisted use	$35.00 / hr
Nikon Optiphot upright microscope
Spot II digital camera for bright field, dark field, DIC / Nomarski, and widefield fluorescence
• Base usage fee for trained clients	$5.00 / hr
• Initial training (~30 min) or assisted use	$35.00 / hr
Leica MZFLIII Stereoscope
Optronics CCD camera, epi-fluorescence, and bright field with MagnaFire acquisition software
• Base usage fee for trained clients	$1.00 / hr
• Initial training (~15 min) or assisted use	$31.00 / hr
Leica DMI4000B with integrated Eppendorf Microinjection System
Inverted microscope with brightfield, widefield fluorescence, and fully-automated DIC and Phase Contrast. Image capture with 1.4 megapixel 12-bit, cooled CCD camera from Q-Imaging.
• Base usage fee for trained clients	$17.50 / hr
• Initial training (~30 min for microscope only) or assisted use	$47.50 / hr
Molecular Devices ArcturusXT laser capture microdissection system
Microdissection/capture of tissue with UV and IR lasers. Brightfield, phase contrast, DIC/Nomarski, and fluorescence microscopy and color CCD camera.
• Base usage fee for trained clients	$10 / hr
• Initial training or assisted use	$40 / hr
Image Processing
Photoshop, Metamorph, AutoQuant, Imaris
High-end computer workstations available for image processing/analysis and figure preparation including deconvolution for blur reduction, 3D rendering, movies/animation, and analytical software for image-based measurements and morphometry.
• Base usage fee for trained clients	$5.00 / hr
• Initial training or assited use	$35.00/ hr
• Deconvolution batch processing (after hours)	$10.00/ evening
Tissue Sectioning
Cryostat
Reichert 2800N Frigocut yields 2-100 um-thick frozen sections
• Base fee for trained users	$5.00 / hr
• Initial training (duration depends on application, includes use fee)	$30.00/ hr
• TBS Tissue Freezing Medium	$20.00 / bottle
• Blades	$3.00 ea
Paraffin microtome
1-50 um-thick paraffin-embedded sections
• Base fee for trained users	$2.00 / hr
• Initial training (duration depends on application, includes use fee)	$15.00/ hr
• Blades	$3.00 ea
• De-waxing paraffin sections on slides	$6.00 / batch
Ultra microtome
Reichert Ultracut S generates 0.1-2.5 um-thick plastic sections for light and electron microscopy
• Base fee for trained users	$3.00 / hr
• Initial training (duration depends on application, includes use fee)	$40.00/ hr
• Use of LKB glass knife maker (glass strips included)	$4.00 / strip
Sample Preparation
• MOWIOL mounting medium (1.5 ml)	$2.00
• Cover slips- various sizes (#1.5 thickness)	$10.00 / box
• Slides	$20.00 / box
•Micro-O-Protect (1 ml)	$10.00
• BSA (10 ml)	$25.00
• BSAc (1 ml)	$6.00
• Cold Water Fish Skin Gelatin (1 ml)	$4.00
• Normal Serum (goat/donkey-1 ml)	$5.00
Miscellaneous
• 700 MB CD-R	$1.00
• 4.7 GB DVD+R	$1.00
•Consultation with Core staff: detailed discussion of experimental design/execution, and/or demonstration or assistance (first hour free)	$30.00
Invitrogen Supply Center
The Cytology Core has an on-site Invitrogen Supply Center stocked with a wide variety of fluorescent markers. In addition to our normal inventory, clients may special order anything from the Invitrogen catalog for no additional cost and without shipping fees.