Zeiss LSM 510 META

The Zeiss LSM 510 META NLO two-photon point-scanning confocal microscope has the ability to simultaneously image up to 8 fluorochromes in a sample, effectively subtract the autofluorescence from the original signal using linear unmixing, spectrally separate two or three closely related fluorophores, perform 3D tile scans covering large sample areas in multiple channels and view specimens with increased penetration (depth along Z axis) along with uniform intensity in samples such as brain slices and whole mounts using the two-photon laser. The system has visible laser lines for fluorophore excitation at 458 nm, 477 nm, 488 nm, 514 nm, 543 nm, and 633 nm. The Chameleon two-photon laser system is a pulsed laser tuneable to any wavelength between 730 nm and 930 nm; this laser can excite most fluorophores in the visible spectrum, but is most efficient with UV and near-UV excitable dyes .

The following objectives are available for this system:

Mag.	Objective Description	NA	Immersion Medium	Working distance (mm)
5x	Plan-Apochromat	0.16	Air	12.1
10x	EC Plan-NeoFluar	0.3	Air	5.6
20x	Plan-Apochromat	0.75	Air	0.61
20x	Plan-Apochromat	0.8	Air	0.55
25x	LCI Plan-NeoFluar	0.8	Water/glycerol	0.21
40x	LD Plan-NeoFluar	0.6	Air	2.5-3.3
40x	EC Plan-NeoFluar	1.3	Oil	0.2
40x	C-Apochromat	1.2	Water	0.28
63x	Plan-Apochromat	1.4	Oil	0.18
63x	C-Apochromat	1.2	Water	0.28
100x	Plan-Apochromat	1.4	Oil	0.17

Check the Schedule here.

The following are meant for reference only and not as substitutes for training and guidance from MCC staff:

Download user instructions here.

Download instructions on spectral unmixing here.