Zeiss LSM 5 LIVE

The Zeiss LSM 5 LIVE line-scanning confocal microscope is designed for ultra-fast 3D live cell imaging but can also image fixed samples at high resolution and higher speed than conventional point-scanning confocals. It can acquire up to 120 frames per second at 512 x 512 pixels (max. resolution is 1536 x 1536) and 70 planes per second for 3D imaging (z-stacking).

This system is enclosed in an incubator box for CO2 and temperature control during extended imaging of cultured cells. It is also equipped with a motorized stage for 3D tile scans covering large sample volumes.

The LIVE has 4 laser lines for fluorescence excitation (405, 488, 532, and 635 nm) and can also perform transmitted light imaging including DIC/Nomarski.

The following objectives are available for this system:

Mag.	Objective Description	NA	Immersion Medium	Working distance (mm)
5x	Plan-Apochromat	0.16	Air	12.1
10x	EC Plan-NeoFluar	0.3	Air	5.6
20x	Plan-Apochromat	0.75	Air	0.61
20x	Plan-Apochromat	0.8	Air	0.55
25x	LCI Plan-NeoFluar	0.8	Water/glycerol	0.21
40x	LD Plan-NeoFluar	0.6	Air	2.5-3.3
40x	EC Plan-NeoFluar	1.3	Oil	0.2
40x	C-Apochromat	1.2	Water	0.28
63x	Plan-Apochromat	1.4	Oil	0.18
63x	C-Apochromat	1.2	Water	0.28
100x	Plan-Apochromat	1.4	Oil	0.17

Check the Schedule here.