MCCF

 

 

 

2

 

 

 

Olympus IX70 inverted microscope with Orca ER digital camera

 

 

An inverted Olympus IX70 is equipped with bright field, phase, DIC, and fluorescence optics.  An excitation filter wheel and focus motor allow rapid acquisition of multi-channel images in 3 dimensions.  System control and image acquisition are done with MetaMorph software.  This system was recently upgraded with a Hamamatsu Orca ER CCD camera, a high sensitivity, low noise device for high resolution imaging.

 

 

 

 

 

Check the Schedule here.

 

 

Download user instructions here (meant for reference only and not as a substitute for training and guidance from MCC staff).

 

 

The following objectives are available for this system:

 

 

Mag.

Objective Description 

NA

Immersion Medium 

μm per pixel

Field of View (μm)

4x

Plan Achromat 

0.1

Air

1.607963

2161.1 x 1646.6

4x

UPlan Apochromat

0.16

Air

1.605505

2157.7 x 1644.0

10x

UPlan Fluor

0.3

Air

0.642307

863.2 x 657.7

20x

UPlanApo

0.7

Air

0.321027

431.4 x 328.7

20x

LWD LC Plan Fluor

0.4

Air

0.322579

433.5 x 330.3

40x

U Apo/340

1.35

Oil

0.160858

216.8 x 165.2

40x

LWD LC Plan Fluor

0.6

Air

0.161290

216.2 x 164.7

60x

60x Plan Apo

1.4

Oil

0.107991

140.0 x 106.7

60x

UPlan Apo

1.2

Water

0.107066

143.9 x 109.6

60x

LWD LC Plan Fluor

0.7

Air

0.104167

145.1 x 110.6

 

 

 

 

 

 

 

 

The following filters from Chroma are available. Click on the name to see the specifications on the Chroma website.

 

 

DAPI #31000

CYAN GFP #31044

ET CYAN #49001

CHAM 2 #71007a

ET GFP #49002

HQ FITC #41001

LUCIFER YELLOW #31010

YELLOW GFP BP #41028

ET YFP #49003

BFP / GFP #51014

HQ TRITC #41002b

HQ CY3 #41007a

CY5 #41008

ET HQ: RdsR #49005

HQ R&B PHYCOERYTHRIN #41003

 

DAPI / RHODAMINE #51001

DAPI / FITC / TEXAS RED #61002

DAPI / FITC / RHODAMINE #61000v2