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The upright Nikon Optiphot 2 is equipped with bright field, phase, DIC, dark field, and fluorescence optics.
The following objectives are available for this microscope:
| Mag. | NA | Description |
| 4x | 0.2 | PlanApo |
| 10x | 0.25 | DL Ph 1 |
| 10x | 0.45 | PlanApo |
| 20x | 0.75 | PlanApo |
| 40x | 0.65 | DL Ph 3 |