Molecular Devices ArcturusXT laser capture microdissection system

An automated system for UV laser cutting (microdissection) and IR laser capture of tissue samples, mounted on a Nikon Eclipse TE2000 inverted microscope. Capable of brightfield, phase-contrast, DIC, and fluorescence microscopy. Equipped with a color CCD camera.

Molecular Devices is currently offering special pricing on LCM consumables to MU researchers! Product information can be found here. For more information, including prices, please contact the MCC or Joseph Pham (Molecular Devices) at 800-635-5577 ext. 3586 or Joseph.Pham@moldev.com.

This system is equipped with five objective lenses:

The following fluorescence filters are available on this system (excitation, emission):

Mag.	NA	Description	340-390, >410 (e.g. DAPI, Hoechst)
2×	0.06	Plan UW	455-495, >510 (e.g. FITC, GFPs, Alexa 488)
4×	0.13	Plan Fluor PhL DL	503-548, >565 (e.g. Cy3, rhodamine, RFPs, Alexa 543)
10×	0.30	Plan Fluor Ph1 DL	570-630, >655 (e.g. Cy5, Alexa 633)
40×	0.60	ELWD Plan Fluor Ph corr.
60×	0.70	ELWD Plan Fluor Ph corr.

Check the Schedule here.

ArcturusXT user guide (for reference only, not a substitute for training and guidance from MCC staff)

Protocols from Molecular Devices:

Generating Cy3 or Cy5-labeled cDNA Probe from Amplified aRNA for Microarray Hybridizations Using A Direct Incorporation Method

Laser Capture Microdissection of Single Chromosomes from Metaphase Spread for Molecular Analysis

Laser Microdissection of Bone Marrow Cells for Molecular Analysis

Laser Microdissection of Fluorescently Stained Drosophila Embryos

Laser Microdissection of Live Plant Cells for Molecular Analysis

Laser Microdissection of Stomatal Cells from Rice Leaf Sections for Molecular Analysis

Optimized Protocol for Mounting Tissue Sections onto Metal-Framed PEN Membrane Slides

Optimized Protocol for Preparing and Staining LCM Samples from Frozen Tissue and Extraction of High-Quality RNA

Optimized protocol for laser microdissection of living in vitro cells

Tissue Scrape Protocol for Verifying RNA Quality Using the PicoPure™ RNA Isolation Kit

Application Notes from Molecular Devices:

A Novel Process for Gene Expression Profiling of Rat and Mouse Tissues from Formalin-Fixed Paraffin-Embedded Sections Using Microarrays

Differential Gene Expression Analysis of Pure Cell Populations from Frozen Uterine Tissue Using Laser Capture Microdissection (LCM) and cDNA Microarrays

Genomic Analysis of Intestinal Responses to Gut Flora Altered by Antimicrobials in Human Flora-Associated (HFA) Mice

Laser Capture Microdissection (LCM) and Expression Profiling of Long-Range Projection Neurons

Laser Capture Microdissection (LCM) for the Analysis of Macrophage Gene Expression from Atherosclerotic Lesions

Laser Capture Microdissection of Cells Labeled with Enhanced Green Fluorescent Protein

Laser Capture Microdissection of muscle fiber populations and expression analysis by RT-PCR

Microgenomic Expression Profiling

Optimized Isolation of Live Plant Tissue using Laser Microdissection for use in Gene Expression Analysis

Veritas microdissection system: optimized protocol for laser microdissection of living in vitro cells